Zeiss LSM700

Model Information

• Zeiss AxioObserver with LSM700 confocal module
• Cite in your manuscripts (example text): NIH Grant S10OD016374
  (Institute:Office of the Director [OD], Office of Research Infrastructure Programs [ORIP])
  Installed: Dec 11, 2013

Specifications:

• Zeiss AxioObserver inverted microscope with LSM700 confocal module
• Portable 37°C heater for 35mm dishes or chamber slides also available
• NA 0.55 condenser (dry only) with DIC for 20X, 40X, 63X and 100X lenses
• Objectives:
  • 10X/0.30 PlanNeofluar (dry, no DIC)
  • 20X/0.80 Planapo (dry, no DIC)
  • 40X/1.30 PlanNeofluar oil, with DIC
  • 40X/1.20 C-Apo water, with DIC, upon request
  • 63X/1.4 PlanApo oil, with DIC
  • 100X PlanApo oil, with DIC, upon request
• 4 laser lines available:
  • 405 (diode laser),
  • 488 (solid-state)
  • 561 (solid-state)
  • 639 (diode laser)
• X-Cite 100W metal-halide illuminator for wide-field fluorescence
• Fluorescence filter sets (wide-field; non-confocal):
  • #49 for DAPI, #38 for GFP, #43 For Cy3
• Latest Zen software to control microscope and confocal
• Zen software on a separate workstation for image analysis.
• Download Zen LE software available for desktop computers in your lab
  • Analysis with Volocity, Imaris or ImageJ on our analysis workstations
  • Use LOCI BioFormats to import images into ImageJ

Applications

• Single confocal images or image stacks for 3D viewing
• FRAP with user-defined photobleach marks
• Photoactivation at 405 nm of caged dyes and other molecules
• Colocalization of different fluorescent signals
• Speed and versatility