Nikon Eclipse Ti2-E with Yokogawa CSU-W1 SoRA Super-Resolution Spinning Disk Confocal
Model Information & Grant Information
- Nikon Eclipse Ti2-E Inverted Microscope Body with Yokogawa CSU-W1 SoRA Super-Resolution Spinning Disk Confocal
- Cite in your manuscripts (example text): NIH Grant S10OD034393
(Institute: Office of the Director [OD])
Installed: July 24, 2023
Specifications
Applications
- Widefield or TIRF fluorescence imaging of membrane proteins, receptor proteins, signalling molecules, cortical cytoskeleton proteins
Special Capabilites
- TIRF illumination exciting molecules within 100 nm of the coverslip surface
- integrated Perfect Focus System to address focus drift with time-lapse imaging
- Photometrics Evolve EMCCD camera: Absolute sensitivity calibration (photon-counting), single-molecule detection with Quant-view and Background Event Reduction Technology (BERT)
- Simultaneous acquisition of two spatially identical but spectrally distinct images using Photometrics Dual-View
System Information
Microscope Specifications
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Objectives
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| Installed | 20x/0.45 S Plan Fluor (dry) |
| 40x/0.60Ph2 S Plan Fluor (dry) | |
| 100x/1.49 Apo TIRF (oil) | |
| Available by request | 40x/1.3 Plan Fluor (oil) 60x/1.4 Plan-Apo (oil) 100x/1.4 Plan-Apo (oil) |
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Filter sets
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| Eyepiece View | CFP |
| YFP | |
| Camera View (both TIRF & Widefield, filter-wheel on emission) |
CFP, YFP, CFP-YFP FRET |
| GFP, RFP, GFP-RFP FRET | |
| Dual-View | CFP/YFP |
| GFP/RFP | |
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Laser Lines
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| CFP | 445nm (40mW Diode laser) |
| not standard cube | 457nm (Argon laser) |
| not standard cube | 477nm (Argon laser) |
| GFP | 488nm (Argon laser) |
| YFP | 514nm (Argon laser) |
| RFP/dSTORM | 561nm (0.5W fiber laser) |
| AF647/dSTORM | 647nm (0.5W fiber laser) |