Follow the 1-page QuickStart Tutorial or the comprehensive 6-page Extras Tutorial to set up your profile and make reservations for the equipment. If you have questions or need assistance using the new scheduler, email microscopy@jhmi.edu.
This week, the 3i Spinning disk is being decommissioned in preparation for the new Nikon Super-res SoRa (underline link to our old/new webpage with our custom specs). We hope to make the new system available to users by early August. In the interim, we have installed the Tokai incubator on our existing Nikon Spinning Disk Confocal (with Slidebook software). We can train you on the Nikon SDC/Slidebook free of charge so that you can image there as soon as you are certified.
You may also elect to be trained on the new Nikon Super-res SoRa (with NIS-Elements software). If you are unsure which microscope you would prefer use, email microscopy@jhmi.edu to schedule a free consultation. We apologize for the late notification, but we look forward to seeing your images on these Nikon scopes.
As you may already be aware, the 2-photon laser has been having stability and power issues. We have been making arrangements for repair/replacement; however, the laser has recently deteriorated to the point of unreliability. This means, that while the microscope is fine for confocal imaging, you should not count on 2-photon and SHG capability for some time.
We apologize for the inconvenience and will update you as the details for repair timeline become clearer.
Update: A new scanner has arrived, and our Zeiss service engineer would like to install it next Tuesday morning. This galvo replacement will completely repair the image quality problems that the LSM780 has been experiencing.
Thank you all for your patience.
Recently, there have been worsening issues with the galvo scanner on the LSM780. This shows up as a distortion or shifting of the image along the X-axis, which is particularly obvious during Live mode or timelapse acquisition.
Zeiss indicates that the scanner needs to be replaced. Unfortunately, it is on backorder, and we do not have an ETA for its arrival from Germany.
Workaround: In the meantime, Zeiss recommends that users reduce scanning speed or rotate the scan area 90 degrees. These remedies might work for you.
Future: If the problem continues to worsen, we will have to remove the microscope from service until the parts arrive.
Alternatives: If Zeiss’s recommendations don’t work for you, we would be happy to discuss alternative approaches, perhaps setting up training sessions on our other Zeiss confocals. Unfortunately, they too are quite busy.
If you need to cancel a LSM780 reservation because of poor instrument performance, just email Micfac.
We hope to notify you of a definite repair date soon. Thank you for your understanding.
At this time, the Leica SDC is decommissioned. The Leica Microscope body had operation issues (i.e., changing objectives, changing from eye/ocular to camera). The spinning disk will be set up in the same room at another table with a new microscope body. The computer and the software will be the same Slidebook software. We will offer free trainings to migrate Leica SDC users to the new system. Thank you for your patience.